Hemocytometer Cell Count

Cells per mL from squares counted; optional trypan blue viability percent.

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Formula and source

cells/mL = (total_cells / squares_counted) * 10^4 * dilution_factor. viability_pct = (total - dead) / total * 100.

Standard improved Neubauer hemocytometer; each large corner square = 1 mm x 1 mm x 0.1 mm = 0.1 uL.

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