PCR Master Mix

Per-component master-mix volume for N reactions with a pipetting fudge factor.

Example

You enter

Number of reactions 24
Fudge factor (%) 10
Components (name,uL_per_reaction per line) name 2x Master Mix, per reaction 12.5, and 4 more

You get

Details, formula, and sources

scaling_factor = number_of_reactions * (1 + fudge_factor_pct / 100). component_total = component_per_reaction * scaling_factor.

Standard master-mix arithmetic. Pipetting fudge factor (default 10%) accounts for dead-volume losses.

No code citation required.

Verify protocol against your lab's SOP before pipetting. A miscalculated dilution can ruin a run or a sample.

Field names used by the API: number_of_reactions, fudge_factor_pct, components, total_per_reaction, total_master_mix, scaling_factor

Fudge factor default 10% (typical for 20-50 reactions)convention; lower for >100 reactions, higher for <12

Details, formula, and sources

scaling_factor = number_of_reactions * (1 + fudge_factor_pct / 100). component_total = component_per_reaction * scaling_factor.

Standard master-mix arithmetic. Pipetting fudge factor (default 10%) accounts for dead-volume losses.

No code citation required.

Verify protocol against your lab's SOP before pipetting. A miscalculated dilution can ruin a run or a sample.

Fudge factor default 10% (typical for 20-50 reactions)convention; lower for >100 reactions, higher for <12