PCR Master Mix

Per-component master-mix volume for N reactions with a pipetting fudge factor.

Run the calculator

Formula and source

scaling_factor = number_of_reactions * (1 + fudge_factor_pct / 100). component_total = component_per_reaction * scaling_factor.

Standard master-mix arithmetic. Pipetting fudge factor (default 10%) accounts for dead-volume losses.

Audience

This tile is built for laboratory and the adjacent professions in the Bench Science and Laboratory Math group. The interactive calculator runs entirely in your browser. No account, no fee, no advertising, no tracking.

Related tools

Posture

Rough Logic answers the math question the working professional asks on the job. The site is a calm, fast, ad-free, account-free, ever-free reference. It does not interpret code. It does not replace the licensed professional. It does not store your inputs. The Authority Having Jurisdiction governs all installations and inspections.