Agarose Gel Percent
Recommended agarose percent for a fragment-size range and grams of agarose for the buffer volume (percent/100 x mL). Per Sambrook & Russell resolution tables; lab SOP governs.
Formula and source
Recommended percent from the fragment-size resolution map (0.5% for >= 20 kb down to 2% for < 1 kb); grams = used percent / 100 x buffer volume (mL); percent clamped to 0.5-3%.
Sambrook & Russell, Molecular Cloning, gel-electrophoresis resolution tables, by name.
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