Nucleic Acid Concentration (A260)
DNA/RNA concentration from a 260 nm read using the mass extinction factor (50 dsDNA, 33 ssDNA/oligo, 40 RNA) and dilution, plus the 260/280 purity ratio that flags protein or phenol carryover. Off every NanoDrop or cuvette read.
Formula and source
concentration = A260 x factor x dilution (factor 50 dsDNA, 33 ssDNA/oligo, 40 RNA, ug/mL per A260); purity_260_280 = A260 / A280.
A260 nucleic-acid quantitation (Beer-Lambert at 260 nm; standard spectrophotometric mass factors), by name.
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