OD600 to Cell Density
Cells/mL = OD600 x conversion factor x dilution, with a linear-range flag above OD ~0.8. Conversion factor is strain- and instrument-specific (user-supplied). Lab SOP governs.
Formula and source
cells/mL = OD600 * factor * dilution, where the OD-to-cells factor is strain- and instrument-specific. Linear range is typically OD < ~0.8 (dilute and re-read above it).
Standard microbiology spectrophotometry; the OD600-to-cell-density relation, by practice.
Audience
This tile is built for laboratory and the adjacent professions in the Bench Science and Laboratory Math group. The interactive calculator runs entirely in your browser. No account, no fee, no advertising, no tracking.
Related tools
Posture
Rough Logic answers the math question the working professional asks on the job. The site is a calm, fast, ad-free, account-free, ever-free reference. It does not interpret code. It does not replace the licensed professional. It does not store your inputs. The Authority Having Jurisdiction governs all installations and inspections.